CDC25C Monoclonal antibody

CDC25C Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2B6B12

Reviews (1)

Cat no : 66912-1-Ig

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3

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Validation Data Gallery

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  WB analysis using 66912-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66912-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66912-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 66912-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below

Product Information

66912-1-Ig targets CDC25C in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDC25C fusion protein Ag10240
• Full Name: cell division cycle 25 homolog C (S. pombe)
• Calculated molecular weight: 473 aa, 53 kDa
• Observed molecular weight: 46-53 kDa
• GenBank accession number: BC019089
• Gene symbol: CDC25C
• Gene ID (NCBI): 995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Publications

Species	Application	Title
mouse	WB	Reprod Toxicol; High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.; Authors - Yi-Ming Ji
mouse	WB	Oncol Lett; Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis.; Authors - Jun Ma
human	WB	Food Chem Toxicol; DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells; Authors - Silu Hou
mouse	WB	Toxicology; ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice; Authors - Ming Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angélique (Verified Customer) (11-25-2021)

on 20ug total proteins

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: murine hepatocytes

CDC25C Antibody Western Blot validation (1/2000 dilution) in murine hepatocytes (Cat no:66912-1-Ig)