CDC25C Monoclonal antibody
CDC25C Monoclonal Antibody for FC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2B6B12
Cat no : 66912-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells |
Positive FC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
Product Information
66912-1-Ig targets CDC25C in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDC25C fusion protein Ag10240 |
Full Name | cell division cycle 25 homolog C (S. pombe) |
Calculated molecular weight | 473 aa, 53 kDa |
Observed molecular weight | 46-53 kDa |
GenBank accession number | BC019089 |
Gene symbol | CDC25C |
Gene ID (NCBI) | 995 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CDC25C antibody 66912-1-Ig | Download protocol |
FC protocol for CDC25C antibody 66912-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Reprod Toxicol High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation. | ||
Oncol Lett Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis. | ||
Food Chem Toxicol DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells | ||
Toxicology ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Angélique (Verified Customer) (11-25-2021) | on 20ug total proteins
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