CDC25C Monoclonal antibody

CDC25C Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat and More (2)







Cat no : 66912-1-Ig


CDC25, CDC25C, M phase inducer phosphatase 3

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Raji cells
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

66912-1-Ig targets CDC25C in WB, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC25C fusion protein Ag10240
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated Molecular Weight 473 aa, 53 kDa
Observed Molecular Weight 46-53 kDa
GenBank Accession NumberBC019089
Gene Symbol CDC25C
Gene ID (NCBI) 995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.


Product Specific Protocols
WB protocol for CDC25C antibody 66912-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Reprod Toxicol

High-dose zearalenone exposure disturbs G2/M transition during mouse oocyte maturation.

Authors - Yi-Ming Ji

Oncol Lett

Luteolin potentiates low-dose oxaliplatin-induced inhibitory effects on cell proliferation in gastric cancer by inducing G2/M cell cycle arrest and apoptosis.

Authors - Jun Ma

Food Chem Toxicol

DON entry into the nucleus induces DNA damage, apoptosis and cycle arrest in GES-1 cells

Authors - Silu Hou


ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice

Authors - Ming Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Angélique (Verified Customer) (11-25-2021)

on 20ug total proteins

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: murine hepatocytes
CDC25C Antibody Western Blot validation (1/2000 dilution) in murine hepatocytes (Cat no:66912-1-Ig)