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CoraLite® Plus 488-conjugated CDC25C Monoclonal antibody

CDC25C Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B6B12

Cat no : CL488-66912

Synonyms

CDC25, CDC25C, M phase inducer phosphatase 3

Validation Data Gallery

Applications:
  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated CDC25C Monoclonal an (CL488-66912)

    FC experiment of K-562 using CL488-66912

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC25C (CL488-66912, Clone:2B6B12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-66912 targets CDC25C in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDC25C fusion protein Ag10240
Full Name cell division cycle 25 homolog C (S. pombe)
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight 46-53 kDa
GenBank accession numberBC019089
Gene symbol CDC25C
Gene ID (NCBI) 995
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CDC25C antibody CL488-66912Download protocol
Standard Protocols
Click here to view our Standard Protocols