CoraLite® Plus 488-conjugated CDC25C Monoclonal antibody
CDC25C Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B6B12
Cat no : CL488-66912
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
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Sample-dependent, check data in validation data gallery |
Product Information
CL488-66912 targets CDC25C in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDC25C fusion protein Ag10240 |
Full Name | cell division cycle 25 homolog C (S. pombe) |
Calculated molecular weight | 473 aa, 53 kDa |
Observed molecular weight | 46-53 kDa |
GenBank accession number | BC019089 |
Gene symbol | CDC25C |
Gene ID (NCBI) | 995 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 488 nm / 515 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cell division cycle 25 (CDC25) family of proteins are highly conserved dual specificity phosphatases that activate cyclin-dependent kinase (CDK) complexes, which in turn regulate progression through the cell division cycle. CDC25C (cell division cycle 25 homolog C) is one of the 3 isoforms of CDC25 in mammalian cells. It is present in the cytoplasm of asynchronously growing human cells (PMID:10330186). And it may be phosphorylated by its own substrate, an active cdc2-cyclin B complex, to create an autoactivation loop (PMID:7937793). It can be hyperphosphorylated to get the active form of 70 kDa (PMID:18296513). CDC25C has some isofroms with molecular mass of 46-53 kDa.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 CDC25C antibody CL488-66912 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |