CDC5L Recombinant monoclonal antibody

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  WB analysis using 85788-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85788-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85788-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

CDC5L Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85788-5-RR

Clone No.250089G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U2OS cells, HCT 116 cells, NIH/3T3 cells, Neuro-2a cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:8000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85788-5-RR targets CDC5L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3578

  Product name: Recombinant human CDC5L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 451-803 aa of BC001568

  Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

• Full Name: CDC5 cell division cycle 5-like (S. pombe)
• Calculated Molecular Weight: 802 aa, 92 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001568
• Gene Symbol: CDC5L
• Gene ID (NCBI): 988
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99459
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.