CoraLite® Plus 488-conjugated CDC5L Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-85788-5

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CDC5L antibody (CL488-85788-5, Clone: 250089G5 ) at dilution of 1:200, CL594-Phalloidin (red).

CDC5L Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85788-5

Clone No.250089G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85788-5 targets CDC5L in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3578

  Product name: Recombinant human CDC5L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 451-803 aa of BC001568

  Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

• Full Name: CDC5 cell division cycle 5-like (S. pombe)
• Calculated Molecular Weight: 802 aa, 92 kDa
• GenBank Accession Number: BC001568
• Gene Symbol: CDC5L
• Gene ID (NCBI): 988
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99459
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.