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CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 19117-1-AP

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

The immunogen of 19117-1-AP is CDK6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5600
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 19117-1-APDownload protocol
IHC protocol for CDK6 antibody 19117-1-APDownload protocol
IP protocol for CDK6 antibody 19117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Estrogen-induced circRNA, circPGR, functions as a ceRNA to promote estrogen receptor-positive breast cancer cell growth by regulating cell cycle-related genes.

Authors - Lei Wang
humanWB

Cancers (Basel)

A Patient-Derived Xenograft Model of Dedifferentiated Endometrial Carcinoma: A Proof-of-Concept Study for the Identification of New Molecularly Informed Treatment Approaches.

Authors - Chiao-Yun Lin
humanWB

Oncotarget

High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Authors - Delong Meng
humanWB

Front Pharmacol

Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing β-Catenin Expression.

Authors - Yinshan Jin
humanWB

Cancer Med

δ-Catenin regulates proliferation and apoptosis in renal cell carcinoma via promoting β-catenin nuclear localization and activating its downstream target genes.

Authors - Lincheng Ju
mouseWB

Onco Targets Ther

A Novel lncRNA ENST00000512916 Facilitates Cell Proliferation, Migration and Cell Cycle Progression in Ameloblastoma.

Authors - Yan Sun