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CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19117-1-AP


CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HeLa cells, C6 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

The immunogen of 19117-1-AP is CDK6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5600
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).


Product Specific Protocols
WB protocol for CDK6 antibody 19117-1-APDownload protocol
IHC protocol for CDK6 antibody 19117-1-APDownload protocol
IP protocol for CDK6 antibody 19117-1-APDownload protocol
FC protocol for CDK6 antibody 19117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biotechnol Appl Biochem

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Authors - Hui Li

Cancer Biother Radiopharm

γ-Glutamylcyclotransferase Knockdown Inhibits Growth of Lung Cancer Cells Through G0/G1 Phase Arrest.

Authors - Zhifeng Lin

Cell Death Dis

GLI1 reduces drug sensitivity by regulating cell cycle through PI3K/AKT/GSK3/CDK pathway in acute myeloid leukemia.

Authors - Cheng Zhou

J Ophthalmol

LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Authors - Yong Wang

Onco Targets Ther

SUN1 silencing inhibits cell growth through G0/G1 phase arrest in lung adenocarcinoma.

Authors - Weiyi Huang

Onco Targets Ther

A Novel lncRNA ENST00000512916 Facilitates Cell Proliferation, Migration and Cell Cycle Progression in Ameloblastoma.

Authors - Yan Sun