CEP152 Polyclonal antibody, PBS Only

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• CEP152 Polyclonal antibody, PBS Only (21815-1-PBS)

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  WB analysis using 21815-1-AP (same clone as 21815-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

  IP result of anti-CEP152 (IP:21815-1-AP, 4ug; Detection:21815-1-AP 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CEP152 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21815-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0912, centrosomal protein 152kDa, CEP 152

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Product Information

21815-1-PBS targets CEP152 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: centrosomal protein 152kDa
• Calculated Molecular Weight: 189 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_014985
• Gene Symbol: CEP152
• Gene ID (NCBI): 22995
• RRID: AB_2878921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94986
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP152, also named as KIAA0912, is a regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. It is necessary for centrosome duplication. CEP152 functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation. CEP152, ATR and CENPJ control cellular responses to DNA damage. CEP152 has some isoforms with MW 189 kDa, 152 kDa, 110 kDa and 66 kDa.