CEP44 Polyclonal antibody, PBS Only

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• CEP44 Polyclonal antibody, PBS Only (24457-1-PBS)

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  WB analysis using 24457-1-AP (same clone as 24457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 24457-1-AP (same clone as 24457-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24457-1-AP (same clone as 24457-1-PBS)

  IP result of anti-CEP44 (IP:24457-1-AP, 4ug; Detection:24457-1-AP 1:3000) with HeLa cells lysate 1640 ug. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 24457-1-AP (same clone as 24457-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP44 antibody (24457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CEP44 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24457-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Centrosomal protein of 44 kDa, HBV PreS1-transactivated protein 3, PS1TP3

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Product Information

24457-1-PBS targets CEP44 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19961

  Product name: Recombinant human KIAA1712 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC038667

  Sequence: MATGDLKRSLRNLEQVLRLLNYPEEVDCVGLIKGDPAASLPIISYSFTSYSPYVTELIMESNVELIAKNDLRFIDAVYKLLRDQFNYKPILTKKQFIQCGFAEWKIQIVCDILNCVMKKHKELSSLQKIPSQQRKKISSGKSEPPLGNEKISAEAVGVDISGRFMTSGKKKAVVIRHLYNEDNVDISEDTLSPITDVNEAVDVSDLNATEIKMPEVKVPEIKAEQQDVNVNPEITALQTMLAECQENLKKLTSIEKRLDCLEQKMKGKVMVDENTWTNLLSRVTLLETEMLLSKKNDEFIEFNEVSEDYASCSDMDLLNPHRKSEVERPASIPLSSGYSTASSDSTPRAS

• Full Name: KIAA1712
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC038667
• Gene Symbol: CEP44
• Gene ID (NCBI): 80817
• RRID: AB_2879557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C0F1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.