CFAP70 Polyclonal antibody, PBS Only

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• CFAP70 Polyclonal antibody, PBS Only (31344-1-PBS)

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  IP experiment of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  IP result of anti-CFAP70 (IP:31344-1-AP, 4ug; Detection:31344-1-AP 1:300) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CFAP70 antibody (31344-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

CFAP70 Polyclonal Antibody for IHC, IF-P, IP, Indirect ELISA

Cat No. 31344-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, IP, Indirect ELISA

Synonyms

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Product Information

31344-1-PBS targets CFAP70 in IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35209

  Product name: Recombinant human TTC18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC032856

  Sequence: MEQVPSAGRLVQITVTEGYDLKGFKGDTPVTFIRAEFNQVVLGDSAKITVSPEGSAKYNFTSSFEFNPEGGITSDDLAHKPVFLTVTEVLPKEKKQKEEKTLILGQAVVDLLPLLEGQSSFQTTVPLHPVQGSPLETPRSSAKQCSLEVKVLVAEPLLTTAQISGGNLLKVTLEAAYSVPESFIPTGPGQNYMVGLQVPS

• Full Name: tetratricopeptide repeat domain 18
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 126 kDa
• GenBank Accession Number: BC032856
• Gene Symbol: TTC18
• Gene ID (NCBI): 118491
• RRID: AB_3669954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T0N1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).