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Complement factor B Monoclonal antibody

Complement factor B Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

8D2B6

Cat no : 66154-1-Ig

Synonyms

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Complement factor B Monoclonal antibody (66154-1-Ig)

    WB analysis of human plasma using 66154-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66154-1-Ig (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IHC staining of human liver using 66154-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66154-1-Ig (Complement factor B antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Complement factor B Monoclonal antibody (66154-1-Ig)

    IF Staining of human liver using 66154-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Complement factor B antibody (66154-1-Ig, Clone: 8D2B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66154-1-Ig is Complement factor B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Complement factor B fusion protein Ag19107
Full Name complement factor B
Calculated molecular weight 86 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC007990
Gene symbol CFB
Gene ID (NCBI) 629
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is cleaved into Ba and Bb by factor D in the presence of C3b. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Protocols

Product Specific Protocols
WB protocol for Complement factor B antibody 66154-1-IgDownload protocol
IHC protocol for Complement factor B antibody 66154-1-IgDownload protocol
IF protocol for Complement factor B antibody 66154-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols