CFHR3 Polyclonal antibody, PBS Only

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• CFHR3 Polyclonal antibody, PBS Only (16583-1-PBS)

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  WB analysis using 16583-1-AP (same clone as 16583-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 16583-1-AP (same clone as 16583-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 16583-1-AP (same clone as 16583-1-PBS)

  IP result of anti-CFHR3 (IP:16583-1-AP, 4ug; Detection:16583-1-AP 1:300) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16583-1-AP (same clone as 16583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16583-1-PBS in a different storage buffer formulation.

CFHR3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 16583-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

16583-1-PBS targets CFHR3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9963

  Product name: Recombinant human CFHR3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC058009

  Sequence: MLLLINVILTLWVSCANGQVKPCDFPDIKHGGLFHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDYIHCTQNGWSPAVPCLRKCYFPYLENGYNQNYGRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQPICINSSEKCGPPPPISNGDTTSFLLKVYVPQSRVEYQCQPYYELQGSNYVTCSNGEWSEPPRCIHPCIITEENMNKNNIKLKGRSDRKYYAKTGDTIEFMCKLGYNANTSILSFQAVCREGIVEYPRCE

• Full Name: complement factor H-related 3
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 45-56 kDa
• GenBank Accession Number: BC058009
• Gene Symbol: CFHR3
• Gene ID (NCBI): 10878
• RRID: AB_2080151
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02985
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).