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  • KD/KO Validated

Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10960-1-AP

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18



Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, mouse brain tissue, human skin tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10960-1-AP targets Cofilin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin fusion protein Ag1399
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC012318
Gene symbol CFL1
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Cofilin antibody 10960-1-APDownload protocol
IHC protocol for Cofilin antibody 10960-1-APDownload protocol
IF protocol for Cofilin antibody 10960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Andrologia

Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.

Authors - K Cui
humanIHC

Dis Esophagus

The expression of CFL1 and N-WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features.

Authors - Wang Wei-Sen WS

Oncol Rep

The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy.

Authors - Qiong Wu
mouseWB,IF

Biochem Biophys Res Commun

Rabies virus inactivates cofilin to facilitate viral budding and release.

Authors - Jie Zan
  • KD Validated
monkeyWB

Neural Regen Res

No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Authors - Ruo-Xu Liu
ratWB

Asian J Androl

Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1.

Authors - Kai Cui