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  • KD/KO Validated

Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 10960-1-AP


18 kDa phosphoprotein, CFL, CFL1, Cofilin, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, human skin tissue, human brain tissue, mouse brain tissue, rat brain tissue, NCI-H1299 cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10960-1-AP targets Cofilin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin fusion protein Ag1399
Full Name cofilin 1 (non-muscle)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18-22 kDa
GenBank Accession NumberBC012318
Gene Symbol CFL1
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.


Product Specific Protocols
WB protocol for Cofilin antibody 10960-1-APDownload protocol
IHC protocol for Cofilin antibody 10960-1-APDownload protocol
IF protocol for Cofilin antibody 10960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

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