|Positive WB detected in||MCF-7 cells, NIH/3T3 cells, mouse brain tissue, human skin tissue, human brain tissue, rat brain tissue|
|Positive IHC detected in||human cervical cancer tissue, human lung cancer tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10960-1-AP targets Cofilin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cofilin fusion protein Ag1399|
|Full Name||cofilin 1 (non-muscle)|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18-22 kDa|
|GenBank accession number||BC012318|
|Gene ID (NCBI)||1072|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
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