CHD2 Polyclonal antibody

CHD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12311-1-AP

Synonyms

ATP dependent helicase CHD2, CHD 2, CHD2

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Validation Data Gallery

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  WB analysis of human placenta using 12311-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12311-1-AP (CHD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 12311-1-AP

  IP result of anti-CHD2 (IP:12311-1-AP, 4ug; Detection:12311-1-AP 1:300) with human placenta tissue lysate 4000ug.

• 

  IHC staining of human lymphoma using 12311-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12311-1-AP (CHD2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12311-1-AP targets CHD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHD2 fusion protein Ag2960
• Full Name: chromodomain helicase DNA binding protein 2
• Calculated Molecular Weight: 1828 aa, 211 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC020810
• Gene Symbol: CHD2
• Gene ID (NCBI): 1106
• RRID: AB_2079925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromodomain-helicase-DNA-binding protein 2, also named as ATP-dependent helicase CHD2, CHD2. It is a sequence-selective DNA-binding protein. Molecular mass of CHD2 is 211kD (201kd for isoform 2, 57kd for isoform 3). CHD2 is confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage.

Publications

Species	Application	Title
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
human	WB	PeerJ; Identification and validation of immune related core transcription factors GTF2I in NAFLD.; Authors - Minbo Zhang
human	WB	Cancer Discov; CHD2 Regulates Neuron-glioma Interactions in Pediatric Glioma; Authors - Xu Zhang