Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | rat brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21334-1-AP targets CHD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15429 Product name: Recombinant human CHD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-230 aa of BC020810 Sequence: MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEVPKRQTRRRAAKNV Predict reactive species |
| Full Name | chromodomain helicase DNA binding protein 2 |
| Calculated Molecular Weight | 1828 aa, 211 kDa |
| Observed Molecular Weight | 230-250 kDa |
| GenBank Accession Number | BC020810 |
| Gene Symbol | CHD2 |
| Gene ID (NCBI) | 1106 |
| RRID | AB_3085652 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14647 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Chromodomain helicase DNA binding protein 2 (CHD2), a member of the sucrose nonfermenting (SNF-2) protein family of epigenetic regulators, which use the energy from ATP hydrolysis to change nucleosome positioning, composition and chromatin structure(PMID: 33435571). CHD2 protein plays a critical role in embryonic development, tumor suppression and survival(PMID: 24834135). In addition, CHD2 has been proposed to prevent breast cancer initiation, and CHD2 mutations are associated with chronic lymphocytic leukemia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHD2 antibody 21334-1-AP | Download protocol |
| IHC protocol for CHD2 antibody 21334-1-AP | Download protocol |
| WB protocol for CHD2 antibody 21334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for 21334-1-AP (Chd2 antibody) is a 5-star rating from an immunofluorescence application. The user reported "nice and clean staining" when using the antibody at a 1:100 dilution on cryostat sections of 4% PFA-fixed human brain organoids derived from iPSCs, imaged at 20x magnification with Chd2 in magenta and DAPI in blue. Overall, the feedback is positive, highlighting clear and specific staining performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Raquel (Verified Customer) (07-12-2024) | Nice and clean staining. Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs using 21334-1-AP Chd2 antibody at dilution of 1:100 (under 20x lens). (Magenta: Chd2 ; Blue: DAPI)
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