CoraLite®594-conjugated CHD4 Monoclonal antibody

• Featured Product
• KD/KO Validated

CHD4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9G2

Cat no : CL594-66222

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66222

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CHD4 antibody (CL594-66222, Clone: 3D9G2 ) at dilution of 1:100. Green: Stain with PF00001, CoraLite®488-Phalloidin.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66222 targets CHD4 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CHD4 fusion protein Ag5729
• Full Name: chromodomain helicase DNA binding protein 4
• Calculated Molecular Weight: 1937 aa, 221 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: BC038596
• Gene Symbol: CHD4
• Gene ID (NCBI): 1108
• RRID: AB_2919967
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.