CIB1 Polyclonal antibody, PBS Only

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• CIB1 Polyclonal antibody, PBS Only (11823-1-PBS)

• 

  WB analysis of human liver using 11823-1-AP (same clone as 11823-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11823-1-AP (same clone as 11823-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 11823-1-AP (same clone as 11823-1-PBS)

  IP result of anti-CIB1 (IP:11823-1-AP, 4ug; Detection:11823-1-AP 1:300) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11823-1-AP (same clone as 11823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 11823-1-AP (same clone as 11823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 11823-1-AP (same clone as 11823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 11823-1-AP (same clone as 11823-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CIB1 antibody (11823-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11823-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

11823-1-PBS targets CIB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2391

  Product name: Recombinant human CIB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-191 aa of BC000846

  Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000846
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• RRID: AB_2079679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99828
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.