CIDEB Polyclonal antibody

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  WB analysis using 27600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27600-1-AP (CIDEB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CIDEB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27600-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

Cell death activator CIDE B, CIDEB

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

27600-1-AP targets CIDEB in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIDEB fusion protein Ag27003
• Full Name: cell death-inducing DFFA-like effector b
• Calculated Molecular Weight: 219 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC035970
• Gene Symbol: CIDEB
• Gene ID (NCBI): 27141
• RRID: AB_2880922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHD4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death‐inducing DFFA‐like effector B (CIDEB), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although CIDEA is expressed at high levels in brown adipose tissue (BAT) and CIDEC is highly enriched in white adipose tissue (WAT) and BAT, CIDEB is more abundant in liver, kidney, small intestine, and colon. CIDEB is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li
human	WB	Cancer Biol Ther; The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer; Authors - Wen-Jing Yun
	WB	Int J Mol Sci; Unveiling Novel Mechanism of CIDEB in Fatty Acid Synthesis Through ChIP-Seq and Functional Analysis in Dairy Goat; Authors - Qiuya He