CIDEB Polyclonal antibody

CIDEB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27600-1-AP

Synonyms

Cell death activator CIDE B, CIDEB



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27600-1-AP targets CIDEB in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIDEB fusion protein Ag27003
Full Name cell death-inducing DFFA-like effector b
Calculated molecular weight 219 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC035970
Gene symbol CIDEB
Gene ID (NCBI) 27141
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cell death‐inducing DFFA‐like effector B (Cideb), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although Cidea is expressed at high levels in brown adipose tissue (BAT) and Cidec is highly enriched in white adipose tissue (WAT) and BAT, Cideb is more abundant in liver, kidney, small intestine, and colon. Cideb is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)

Protocols

Product Specific Protocols
WB protocol for CIDEB antibody 27600-1-APDownload protocol
IHC protocol for CIDEB antibody 27600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Hazard Mater

TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.

Authors - Changying Li