CIDEB Polyclonal antibody

CIDEB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27600-1-AP

Synonyms

Cell death activator CIDE B, CIDEB

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Validation Data Gallery

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  WB analysis of HepG2 using 27600-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27600-1-AP (CIDEB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27600-1-AP (CIDEB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

27600-1-AP targets CIDEB in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIDEB fusion protein Ag27003
• Full Name: cell death-inducing DFFA-like effector b
• Calculated molecular weight: 219 aa, 28 kDa
• Observed molecular weight: 28 kDa
• GenBank accession number: BC035970
• Gene symbol: CIDEB
• Gene ID (NCBI): 27141
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death‐inducing DFFA‐like effector B (Cideb), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although Cidea is expressed at high levels in brown adipose tissue (BAT) and Cidec is highly enriched in white adipose tissue (WAT) and BAT, Cideb is more abundant in liver, kidney, small intestine, and colon. Cideb is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li