CIN85 Polyclonal antibody, PBS Only

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• CIN85 Polyclonal antibody, PBS Only (12132-1-PBS)

• 

  WB analysis using 12132-1-AP (same clone as 12132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 12132-1-AP (same clone as 12132-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12132-1-AP (same clone as 12132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12132-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CIN85 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12132-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

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Product Information

12132-1-PBS targets CIN85 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2777

  Product name: Recombinant human CIN85 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC015806

  Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPP

• Full Name: SH3-domain kinase binding protein 1
• Calculated Molecular Weight: 665 aa, 73 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC015806
• Gene Symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• RRID: AB_2187277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96B97
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.