Tested Applications
| Positive WB detected in | rat testis tissue, A375 cells, mouse testis tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human colon cancer tissue, human thyroid cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26999-1-AP targets CITED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24643 Product name: Recombinant human CITED1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC004240 Sequence: MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMQLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSD Predict reactive species |
| Full Name | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC004240 |
| Gene Symbol | CITED1 |
| Gene ID (NCBI) | 4435 |
| RRID | AB_2880718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. The CITED1 gene encodes a 27 kDa protein, but a 30 kDa band was also detected in the publication. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526, 15272279, 15843474).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CITED1 antibody 26999-1-AP | Download protocol |
| IF protocol for CITED1 antibody 26999-1-AP | Download protocol |
| IHC protocol for CITED1 antibody 26999-1-AP | Download protocol |
| IP protocol for CITED1 antibody 26999-1-AP | Download protocol |
| WB protocol for CITED1 antibody 26999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CITED1 Polyclonal antibody (Cat# 26999-1-AP) has 4 total citations. These appear in Science, EMBO Reports, Animals (Basel), and Anim Biosci. The associated research fields include protein degradation via the midnolin-proteasome pathway, TFE3 isoform roles in cell motility and invasion, and rabbit reproductive genetics—specifically MTHFR and CXCL12 as candidate markers for litter size. Applications cited were primarily Western blot, with one study also using immunofluorescence.
| Species | Application | Title |
|---|---|---|
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation | ||
Anim Biosci Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits. |
