CITED1 Polyclonal antibody

CITED1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26999-1-AP

Synonyms

MSG1, Melanocyte-specific protein 1, Cbp/p300-interacting transactivator 1

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Validation Data Gallery

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  WB analysis of rat testis using 26999-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 26999-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26999-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26999-1-AP (CITED1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 26999-1-AP

  IP result of anti-CITED1 (IP:26999-1-AP, 4ug; Detection:26999-1-AP 1:300) with A375 cells lysate 3000ug.

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  IHC staining of human colon cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26999-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26999-1-AP (CITED1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 26999-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CITED1 antibody (26999-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HEK-293T using 26999-1-AP

  1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CITED1 Polyclonal antibody (26999-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: rat testis tissue, A375 cells, mouse testis tissue
• Positive IP detected in: A375 cells
• Positive IHC detected in: human colon cancer tissue, human thyroid cancer tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26999-1-AP targets CITED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CITED1 fusion protein Ag24643
• Full Name: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC004240
• Gene Symbol: CITED1
• Gene ID (NCBI): 4435
• RRID: AB_2880718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CITED1, also named as MSG1, belongs to the CITED family. CITED1 is a bifunctional transcriptional cofactor which is able to activate or repress transcription in association with other transcription factors. The CITED1 gene encodes a 27 kDa protein, but a 30 kDa band was also detected in the publication. The elevation of CITED1 may be directly associated with the preneoplastic phenotype (PMID: 23935526, 15272279, 15843474).

Publications

Species	Application	Title
human	WB	Science; The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation; Authors - Xin Gu
rabbit	WB	Animals (Basel); MTHFR as a Novel Candidate Marker for Litter Size in Rabbits; Authors - Jie Yang

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