Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, A549 cells |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
| Positive FC (Intra) detected in | HeLa cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84485-4-RR targets CKAP4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10022 Product name: Recombinant human CKAP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 254-602 aa of BC082972 Sequence: IFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV Predict reactive species |
| Full Name | cytoskeleton-associated protein 4 |
| Calculated Molecular Weight | 602 aa, 66 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC082972 |
| Gene Symbol | CKAP4 |
| Gene ID (NCBI) | 10970 |
| RRID | AB_3672002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q07065 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CKAP4 antibody 84485-4-RR | Download protocol |
| IF protocol for CKAP4 antibody 84485-4-RR | Download protocol |
| WB protocol for CKAP4 antibody 84485-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CKAP4 Recombinant monoclonal antibody (241671B6), catalog number 84485-4-RR, has 1 total citation. It appears in Molecular and Cellular Biochemistry (IF 4.7), published in 2026. The cited study investigated TMED2's role in macrophage polarization via the MEK/ERK signaling pathway in promoting osteosarcoma progression, placing this citation in the fields of cancer biology, immune cell regulation, and intracellular signaling.
| Species | Application | Title |
|---|---|---|
Mol Cell Biochem TMED2 regulates macrophage polarization through MEK/ERK signaling pathway for osteosarcoma progression promotion.
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