Filter: AllWB

WB analysis using 87517-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 87517-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 87517-1-RR
HEK-293T cells were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 87517-1-RR
mouse heart tissue were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CKMT2 Recombinant monoclonal antibody

Name: CKMT2 Recombinant monoclonal antibody
Brand: Proteintech
SKU: 87517-1-RR

CKMT2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87517-1-RR

Clone No.252920F5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Basic-type mitochondrial creatine kinase, CKMT 2, Creatine kinase S-type, mitochondrial, EC:2.7.3.2, Mib CK

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

rat heart tissue, HEK-293T cells, mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87517-1-RR targets CKMT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3971 Product name: Recombinant human CKMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 57-419 aa of BC029140 Sequence: LRKHNNCMAECLTPAIYAKLRNKVTPNGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHTTDLDASKITQGQFDEHYVLSSRVRTGRSIRGLSLPPACTRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLPQFGKK Predict reactive species
Full Name	creatine kinase, mitochondrial 2 (sarcomeric)
Calculated Molecular Weight	419 aa, 48 kDa
Observed Molecular Weight	41-48 kDa
GenBank Accession Number	BC029140
Gene Symbol	CKMT2
Gene ID (NCBI)	1160
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17540
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKMT2 (Creatine kinase S-type, mitochondrial), also known as s-MtCK, is one of the creatine kinase (CK) isozymes that are tightly coupled to adenosine triphosphate (ATP) output via adenine nucleotide transport proteins or carriers, making it an important player in ATP synthesis and respiratory chain activity (PMID: 38172162). Enhancing Ckmt2 expression or activity can protect against myocardial injury caused by ischemia-reperfusion injury (PMID: 39457679).