CLEC10A/CD301 Recombinant monoclonal antibody

• 

  WB analysis of RAW 264.7 using 85911-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85911-5-RR (CLEC10A/CD301 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CLEC10A/CD301 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85911-5-RR

Clone No.242636A8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Clec10a, C-type lectin domain family 10 member A, Macrophage asialoglycoprotein-binding protein 1, Macrophage galactose/N-acetylgalactosamine-specific lectin, M-ASGP-BP-1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85911-5-RR targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2698

  Product name: Recombinant Mouse MGL1/CD301a protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 57-304 aa of NM_010796.3

  Sequence: QNSQLRRDLGTLRATLDNTTSKIKAEFQSLDSRADSFEKGISSLKVDVEDHRQELQAGRDLSQKVTSLESTVEKREQALKTDLSDLTDHVQQLRKDLKALTCQLANLKNNGSEVACCPLHWTEHEGSCYWFSESEKSWPEADKYCRLENSHLVVVNSLEEQNFLQNRLANVVSWIGLTDQNGPWRWVDGTDFEKGFKNWAPLQPDNWFGHGLGGGEDCAHITTGGPWNDDVCQRTFRWICEMKLAKES

• Full Name: macrophage galactose N-acetyl-galactosamine specific lectin 1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: NM_010796.3
• Gene Symbol: Mgl1
• Gene ID (NCBI): 17312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49300
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).