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CLTC Monoclonal antibody

CLTC Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1


Human, Pig, Mouse, Rat and More (2)







Cat no : 66487-1-Ig


CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034

Tested Applications

Positive WB detected inHeLa cells, Raji cells, Jurkat cells, ROS1728 cells, pig brain tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:80000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66487-1-Ig targets CLTC in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLTC fusion protein Ag25040
Full Name clathrin, heavy chain (Hc)
Calculated molecular weight 192 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC054489
Gene symbol CLTC
Gene ID (NCBI) 1213
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)


Product Specific Protocols
WB protocol for CLTC antibody 66487-1-IgDownload protocol
IHC protocol for CLTC antibody 66487-1-IgDownload protocol
IF protocol for CLTC antibody 66487-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos

Front Microbiol

Porcine Circovirus Type 3 Enters Into PK15 Cells Through Clathrin- and Dynamin-2-Mediated Endocytosis in a Rab5/Rab7 and pH-Dependent Fashion.

Authors - Ruihan Shi