CNPase Polyclonal antibody

CNPase Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13427-1-AP

Synonyms

CNP, CNP1, CNPase



Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13427-1-AP targets CNPase in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNPase fusion protein Ag4227
Full Name 2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight 421 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC028040
Gene symbol CNP
Gene ID (NCBI) 1267
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Protocols

Product Specific Protocols
WB protocol for CNPase antibody 13427-1-APDownload protocol
IHC protocol for CNPase antibody 13427-1-APDownload protocol
IF protocol for CNPase antibody 13427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Physiol (Oxf)

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Authors - Li Zhang
ratWB

Life Sci

Profiling analysis reveals the potential contribution of long non-coding RNAs to preterm white matter injury.

Authors - Dayu Wang
humanWB,IF

PLoS One

2',3'-cyclic nucleotide 3'-phosphodiesterases inhibit hepatitis B virus replication.

Authors - Hui Ma