CNPase Polyclonal antibody

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• CNPase Polyclonal antibody (13427-1-AP)

CNPase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 13427-1-AP Copied!

Publications (27)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA

Synonyms

CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37

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  WB analysis using 13427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13427-1-AP (CNPase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13427-1-AP (CNPase antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 13427-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 13427-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPase (13427-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13427-1-AP targets CNPase in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4227

  Product name: Recombinant human CNP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-421 aa of BC028040

  Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

• Full Name: 2',3'-cyclic nucleotide 3' phosphodiesterase
• Calculated Molecular Weight: 421 aa, 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC028040
• Gene Symbol: CNPase
• Gene ID (NCBI): 1267
• RRID: AB_2082477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Publications

What published studies show

CNPase Polyclonal antibody (Cat# 13427-1-AP) has 27 citations published across 25 journals, including Nature Neuroscience, Nature Communications, Journal of Clinical Investigation, Biomaterials, and Journal of Neurochemistry. Research fields span neurodegeneration, spinal cord injury, white matter injury, oligodendrocyte myelination, neuroinflammation, ferroptosis, and myocardial ischemia/reperfusion injury.

Automatically generated from the 27 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Neurosci; TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.; Authors - Manling Xie
human	WB	Nat Commun; Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA; Authors - Mirjana Malnar Črnigoj
mouse	IF	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	IF	Biomaterials; Novel "hot spring"-mimetic scaffolds for sequential neurovascular network reconstruction and osteoporosis reversion; Authors - Yanan Zhao
rat	IHC	Neural Regen Res; Sodium selenite promotes neurological function recovery after spinal cord injury by inhibiting ferroptosis.; Authors - Yi-Xin Chen
rat	IHC	Neural Regen Res; Mechanisms underlying the promotion of functional recovery by deferoxamine after spinal cord injury in rats; Authors - Jian Hao