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CNPase Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 13427-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

CNPase Antibody IF mouse brain tissue 13427-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CNPase Antibody IF mouse brain tissue 13427-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 13427-1-AP (CNPase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CNPase Antibody WB HepG2 cells 13427-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 13427-1-AP( CNPase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

CNPase Antibody IHC mouse brain tissue 13427-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 13427-1-AP( CNPase antibody at dilution of 1:50 (under 10x lens)

CNPase Antibody IHC mouse brain tissue 13427-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 13427-1-AP( CNPase antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CNPase fusion protein Ag4227
Full name:
2',3'-cyclic nucleotide 3' phosphodiesterase
Calculated molecular weight:
421aa,48 kDa
Observed molecular weight:
45-48 kDa
GenBank accession number:
BC028040
Gene ID (NCBI):
1267
Gene symbol
CNP
Synonyms
CNP, CNP1, CNPase

Background

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.


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