CNPY2/MSAP Polyclonal antibody

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• CNPY2/MSAP Polyclonal antibody (14635-1-AP)

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• KD/KO Validated

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14635-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

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  WB analysis of mouse pancreas using 14635-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14635-1-AP

  WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 14635-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 14635-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 14635-1-AP

  IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 14635-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 14635-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 14635-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14635-1-AP

  Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14635-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 14635-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse pancreas tissue, human liver tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14635-1-AP targets CNPY2/MSAP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6214

  Product name: Recombinant human CNPY2,MSAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-182 aa of BC065015

  Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL

• Full Name: canopy 2 homolog (zebrafish)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 17-21 kDa
• GenBank Accession Number: BC065015
• Gene Symbol: CNPY2
• Gene ID (NCBI): 10330
• RRID: AB_2082621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2B0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

Publications

What published studies show

Anti-CNPY2 affinity-purified antibody (Cat# 14635-1-AP) has 19 total citations published in journals including Nature Structural & Molecular Biology, Journal of Biological Chemistry, Cancer Letters, Apoptosis, Frontiers in Pharmacology, International Journal of Molecular Medicine, and Biochemical Pharmacology. Research fields span oncology (hepatocellular, bladder, gastric, lung cancers), neuroscience (spinal cord injury, Huntington's disease, cerebral ischemia), cardiovascular disease, NAFLD, ER stress/unfolded protein response, and pharmacology.

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cancer Lett; A Novel TLR4 Accessory Molecule Drives Hepatic Oncogenesis through Tumor-Associated Macrophages; Authors - Doyeon Kim
mouse,human	WB	Nat Struct Mol Biol; CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.; Authors - Feng Hong ; KO Validated
human	WB,IF	Apoptosis; Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway; Authors - Chun-Ling Guo ; KD Validated
rat	IHC	Int J Mol Med; Protective effect of Barbaloin in a rat model of myocardial ischemia reperfusion injury through the regulation of the CNPY2‑PERK pathway.; Authors - Yue Cui
rat	WB	Neural Regen Res; Human adipose tissue- and umbilical cord-derived stem cells: which is a better alternative to treat spinal cord injury?; Authors - Ai-Mei Liu
mouse,human	WB,IHC	Biomed Pharmacother; CNPY2 drives DSS-induced colitis via the macrophage-ROS axis; Authors - Wenqing Zhang ; KD Validated