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CNPY2/MSAP Polyclonal antibody, PBS Only (14635-1-PBS)

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
WB analysis using 14635-1-AP (same clone as 14635-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)
WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 14635-1-AP (same clone as 14635-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)
IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 14635-1-AP (same clone as 14635-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 14635-1-AP (same clone as 14635-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)
Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.
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1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CNPY2/MSAP Polyclonal antibody, PBS Only

Name: CNPY2/MSAP Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14635-1-PBS

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 14635-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14635-1-PBS targets CNPY2/MSAP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6214 Product name: Recombinant human CNPY2,MSAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC065015 Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL Predict reactive species
Full Name	canopy 2 homolog (zebrafish)
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	17-21 kDa
GenBank Accession Number	BC065015
Gene Symbol	CNPY2
Gene ID (NCBI)	10330
RRID	AB_2082621
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2B0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.