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Collagen Type III (C-terminal) Polyclonal antibody

Collagen Type III (C-terminal) Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 13548-1-AP

Synonyms

Collagen Type III (C-terminal)



Tested Applications

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13548-1-AP targets Collagen Type III (C-terminal) in WB, IHC, IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type III (C-terminal) fusion protein Ag4229
Full Name collagen, type III, alpha 1
Calculated molecular weight 1466 aa, 139 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC028178
Gene symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three a1(III) chains and is expressed in early embryos and throughout embryogenesis. In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen. COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with type III and IV Ehlers-Danlos syndrome, and with aortic and arterial aneurysms. (PMID: 9050868; 2445760)

Protocols

Product Specific Protocols
WB protocol for Collagen Type III (C-terminal) antibody 13548-1-APDownload protocol
IHC protocol for Collagen Type III (C-terminal) antibody 13548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biomed Mater Res A

Aligned topography mediated cell elongation reverses pathological phenotype of in vitro cultured keloid fibroblasts.

Authors - Jia Huang
ratWB

J Cell Physiol

High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-κB signaling.

Authors - Kaiqun Li
ratIHC

J Cell Mol Med

Rutaecarpine prevents hypertensive cardiac hypertrophy involving the inhibition of Nox4-ROS-ADAM17 pathway.

Authors - Si-Yu Zeng
mouseIHC

Oxid Med Cell Longev

Myricetin Possesses Potential Protective Effects on Diabetic Cardiomyopathy through Inhibiting IκBα/NFκB and Enhancing Nrf2/HO-1.

Authors - Hai-Han Liao
ratWB

J Dev Orig Health Dis

Long-term effects of maternal undernutrition on offspring carotid artery remodeling: role of miR-29c.

Authors - O Khorram
mouseWB

J Forensic Leg Med

A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation.

Authors - Lin-Lin Wang