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COL6A3 Recombinant monoclonal antibody (83010-1-RR)

COL6A3 Recombinant monoclonal antibody

COL6A3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83010-1-RR

Clone No. 230540A11

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

COL6A3-Specific, 230540A11, Collagen alpha 3(VI) chain, Collagen alpha-3(VI) chain, Collagen Type VI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBFC (Intra)Affinity

WB analysis of A375 using 83010-1-RR
A375 cells were subjected to SDS PAGE followed by western blot with 83010-1-RR (COL6A3-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 83010-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug COL6A3-Specific Recombinant antibody (83010-1-RR, Clone:230540A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 83010-1-RR
Biolayer interferometry (BLl) kinetic assays of 83010-1-RR against Human COL6A3-Specific were performed. The affinity constant is 1.63 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A375 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83010-1-RR targets COL6A3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag34752 Product name: Recombinant human COL6A3-Specific protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1630-1850 aa of NM_004369 Sequence: PSRPEKKKADIVFLLDGSINFRRDSFQEVLRFVSEIVDTVYEDGDSIQVGLVQYNSDPTDEFFLKDFSTKRQIIDAINKVVYKGGRHANTKVGLEHLRVNHFVPEAGSRLDQRVPQIAFVITGGKSVEDAQDVSLALTQRGVKVFAVGVRNIDSEEVGKIASNSATAFRVGNVQELSELSEQVLETLHDAMHETLCPGVTDAAKACNLDVILGFDGSRDQN Predict reactive species
Full Name	collagen, type VI, alpha 3
Calculated Molecular Weight	344 kDa
Observed Molecular Weight	300 kDa
GenBank Accession Number	NM_004369
Gene Symbol	COL6A3
Gene ID (NCBI)	1293
RRID	AB_3670754
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P12111
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

Protocols

Product Specific Protocols
FC protocol for COL6A3 antibody 83010-1-RR	Download protocol
WB protocol for COL6A3 antibody 83010-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The COL6A3 Recombinant monoclonal antibody (230540A11), catalog number 83010-1-RR, has 1 total citation. The citation appears in Pathology Research and Practice (Impact Factor 3.7), published in 2026. The associated research field is oncology, specifically investigating COL6A3's role in driving tumor progression and predicting prognosis in cervical squamous cell carcinoma.