CoraLite® Plus 488-conjugated COPA Recombinant monoclonal antibody

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  IF Staining of Hela using CL488-83089-2

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 COPA antibody (CL488-83089-2, Clone: 230361B1 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83089-2

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated COPA Recombinant antibody (CL488-83089-2, Clone:230361B1)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

COPA Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-83089-2

Clone No.230361B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

230361B1, Alpha coat protein, Alpha COP, Coatomer subunit alpha, FLJ26320

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Tested Applications

• Positive IF/ICC detected in: Hela cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83089-2 targets COPA in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34207

  Product name: Recombinant human COPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 925-1224 aa of NM_004371

  Sequence: QLPVDHILAGSFETAMRLLHDQVGVIQFGPYKQLFLQTYARGRTTYQALPCLPSMYGYPNRNWKDAGLKNGVPAVGLKLNDLIQRLQLCYQLTTVGKFEEAVEKFRSILLSVPLLVVDNKQEIAEAQQLITICREYIVGLSVETERKKLPKETLEQQKRICEMAAYFTHSNLQPVHMILVLRTALNLFFKLKNFKTAATFARRLLELGPKPEVAQQTRKILSACEKNPTDAYQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQICRVTTVTEIGKDVIGLRISPLQFR

• Full Name: coatomer protein complex, subunit alpha
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_004371
• Gene Symbol: COPA
• Gene ID (NCBI): 1314
• RRID: AB_3673185
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53621
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COPA, also named HEP-COP and Alpha-COP, is coatomer subunit alpha. The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.