COPS3 Recombinant antibody

COPS3 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 83822-2-RR
Clone No.240918E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3, 240918E3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, HT-29 cells, HEK-293T cells, mouse ovary tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83822-2-RR targets COPS3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COPS3 fusion protein Ag32825 Predict reactive species
Full Name COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001891
Gene Symbol COPS3
Gene ID (NCBI) 8533
RRIDAB_3671406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UNS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Protocols

Product Specific Protocols
WB protocol for COPS3 antibody 83822-2-RRDownload protocol
IHC protocol for COPS3 antibody 83822-2-RRDownload protocol
IF protocol for COPS3 antibody 83822-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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