Ceruloplasmin Antibody

Ceruloplasmin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C8A5

Cat no : 66156-1-Ig

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase



Tested Applications

Positive WB detected inhuman placenta tissue, rat liver tissue, HSC-T6 cells, human kidney tissue, human blood tissue
Positive IHC detected inhuman ovary tumor tissue, human placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66156-1-Ig targets Ceruloplasmin in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535
Full Name ceruloplasmin (ferroxidase)
Calculated molecular weight 1065 aa, 122 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC146663
Gene symbol CP
Gene ID (NCBI) 1356
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Protocols

Product Specific Protocols
WB protocol for Ceruloplasmin antibody 66156-1-IgDownload protocol
IHC protocol for Ceruloplasmin antibody 66156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteomics

PMID: 31706944

Authors - Ni Yang