Ceruloplasmin Monoclonal antibody

Ceruloplasmin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

3C8A5

Cat no : 66156-1-Ig

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

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Validation Data Gallery

• 

  WB analysis of rat liver using 66156-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66156-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66156-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 66156-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 66156-1-Ig

  human blood was subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50.

• 

  IHC staining of human liver using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50.

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66156-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66156-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ceruloplasmin antibody (66156-1-Ig, Clone: 3C8A5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, rat liver tissue, HSC-T6 cells, human kidney tissue, human blood tissue
• Positive IHC detected in: human ovary tumor tissue, human placenta tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

66156-1-Ig targets Ceruloplasmin in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Full Name: ceruloplasmin (ferroxidase)
• Calculated Molecular Weight: 1065 aa, 122 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC146663
• Gene Symbol: CP
• Gene ID (NCBI): 1356
• RRID: AB_2881552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest.; Authors - Marta Zarà
mouse	WB	J Proteomics; PMID: 31706944; Authors - Ni Yang
human	WB, IHC	FEBS Open Bio; Ceruloplasmin overexpression is associated with oncogenic pathways and poorer survival rates in clear-cell renal cell carcinoma.; Authors - Yong Zhang