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CoraLite®594-conjugated Ceruloplasmin Monoclonal antibody

Ceruloplasmin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8A5

Cat no : CL594-66156

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Ceruloplasmin Monoclonal a (CL594-66156)

    IF Staining of HeLa using CL594-66156

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Ceruloplasmin antibody (CL594-66156, Clone: 3C8A5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66156 targets Ceruloplasmin in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ceruloplasmin fusion protein Ag15535
Full Name ceruloplasmin (ferroxidase)
Calculated Molecular Weight 1065 aa, 122 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC146663
Gene Symbol CP
Gene ID (NCBI) 1356
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.

Protocols

Product Specific Protocols
IF protocol for CL594 Ceruloplasmin antibody CL594-66156Download protocol
Standard Protocols
Click here to view our Standard Protocols