CRK Monoclonal antibody

CRK Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2B7E1

Cat no : 67035-1-Ig

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

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Validation Data Gallery

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  WB analysis of HEK-293 using 67035-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67035-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67035-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67035-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67035-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67035-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67035-1-Ig (CRK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Product Information

67035-1-Ig targets CRK in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRK fusion protein Ag28793
• Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC008506
• Gene symbol: CRK
• Gene ID (NCBI): 1398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.