CRK Monoclonal antibody

CRK Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2B7E1

Cat no : 67035-1-Ig

Synonyms

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

67035-1-Ig targets CRK in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRK fusion protein Ag28793
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008506
Gene symbol CRK
Gene ID (NCBI) 1398
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.

Protocols

Product Specific Protocols
WB protocol for CRK antibody 67035-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols