Cryptochrome 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cryptochrome 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (2)

Cat no : 13474-1-AP

Synonyms

CRY1, Cryptochrome-1, CRY 1

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Validation Data Gallery

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  WB analysis using 13474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13474-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 13474-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 13474-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, Y79 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

13474-1-AP targets Cryptochrome 1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cryptochrome 1 fusion protein Ag4270
• Full Name: cryptochrome 1 (photolyase-like)
• Calculated Molecular Weight: 586 aa, 66 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC030519
• Gene Symbol: CRY1
• Gene ID (NCBI): 1407
• RRID: AB_10697652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

Publications

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
mouse	WB	Sci Total Environ; Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice; Authors - Yajie Guan
human	IF	Theranostics; Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.; Authors - Mark A Naven
human	WB	EMBO Rep; Circadian networks in human embryonic stem cell-derived cardiomyocytes.; Authors - Pieterjan Dierickx
human	WB	Stem Cell Reports; SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.; Authors - Bastiaan C Du Pré

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-11-2023)

I have used this for Human cardiomyocytes cell line and mice skin, and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

FH

Iru (Verified Customer) (06-26-2020)

It works fine in 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells