Anti-Mouse IL-3RB/CD131 Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using 98232-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-3RB/CD131 Rabbit Recombinant Antibody (98232-1-RR, Clone: 241954C1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone: M1/70). Cells were not fixed.

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  FC experiment of mouse bone marrow cells using 98232-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-3RB/CD131 Rabbit Recombinant Antibody (98232-1-RR, Clone: 241954C1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98232-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98232-1-RR against Mouse IL-3RB/CD131 were performed. The affinity constant is below 1 pM.

IL-3RB/CD131 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98232-1-RR

Clone No.241954C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Cytokine receptor common subunit beta, Csf2rb1, Csf2rb, CD131, Aic2b

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98232-1-RR targets IL-3RB/CD131 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 99kDa
• GenBank Accession Number: NM_007780.4
• Gene Symbol: IL-3RB/CD131
• Gene ID (NCBI): 12983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26955
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD131, also known as IL-3RB, IL-5RB, colony stimulating factor 2 receptor subunit beta (CSF2RB), or cytokine receptor common subunit beta, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 and IL-5 (PMID: 36532080). It is expressed predominantly by hematopoietic cells but also by non-hematopoietic cells (PMID: 10582335). CD131 mediates intracellular signaling by JAK2, leading to phosphorylation of CD131 and triggering signaling pathways such as STAT5, MAPK and PI3-Kinase/AKT which regulate cell survival, proliferation and function (PMID: 23535386).