CoraLite® Plus 647 Anti-Mouse IL-3RB/CD131 Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using CL647-98232

  1x10^6 mouse bone marrow cells were surface stained with CoraLite® Plus 488 Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 647 Anti-Mouse IL-3RB/CD131 Rabbit RecAb (CL647-98232, Clone: 241954C1) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-98232

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse IL-3RB/CD131 Rabbit RecAb (CL647-98232, Clone: 241954C1) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

IL-3RB/CD131 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL647-98232

Clone No.241954C1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241954C1, Aic2b, CD131, Csf2rb, Csf2rb1

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98232 targets IL-3RB/CD131 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 99kDa
• GenBank Accession Number: NM_007780.4
• Gene Symbol: IL-3RB/CD131
• Gene ID (NCBI): 12983
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26955
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD131, also known as IL-3RB, IL-5RB, colony stimulating factor 2 receptor subunit beta (CSF2RB), or cytokine receptor common subunit beta, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3 and IL-5 (PMID: 36532080). It is expressed predominantly by hematopoietic cells but also by non-hematopoietic cells (PMID: 10582335). CD131 mediates intracellular signaling by JAK2, leading to phosphorylation of CD131 and triggering signaling pathways such as STAT5, MAPK and PI3-Kinase/AKT which regulate cell survival, proliferation and function (PMID: 23535386).