CTAGE5 Polyclonal antibody, PBS Only

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• CTAGE5 Polyclonal antibody, PBS Only (55279-1-PBS)

• 

  WB analysis using 55279-1-AP (same clone as 55279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 55279-1-AP (same clone as 55279-1-PBS)

  WB result of CTAGE5 antibody (55279-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTAGE5 transfected HepG2 cells. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 55279-1-AP (same clone as 55279-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 55279-1-AP (same clone as 55279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 55279-1-AP (same clone as 55279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55279-1-AP (same clone as 55279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CTAGE5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55279-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

cTAGE family member 5, CTAGE family member 5 ER export factor, CTAGE family, member 5, Cutaneous T-cell lymphoma-associated antigen 5, MEA11

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Product Information

55279-1-PBS targets CTAGE5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: CTAGE family, member 5
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_005930
• Gene Symbol: CTAGE5
• Gene ID (NCBI): 4253
• RRID: AB_11183044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PC5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.