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Tested Applications
| Positive FC detected in | PHA treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PY7-98078 targets CTLA-4/CD152 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CTLA-4/CD152 fusion protein Eg0972 Predict reactive species |
| Full Name | cytotoxic T-lymphocyte-associated protein 4 |
| Calculated Molecular Weight | 223 aa, 25 kDa |
| GenBank Accession Number | BC070162 |
| Gene Symbol | CTLA4 |
| Gene ID (NCBI) | 1493 |
| Conjugate | PE-Cyanine7 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 450-500 nm / 778 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P16410 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine7 CTLA-4/CD152 antibody PY7-98078 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
