Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody

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  IHC staining of human tonsillitis using 98078-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98078-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98078-1-RR (CTLA-4/CD152 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

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  FC experiment of human PBMCs using 98078-1-RR

  1x10^6 PHA-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human CTLA-4/CD152 Rabbit RecAb (98078-1-RR, Clone: 240793E8)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 unstimulated human PBMCs were surface stained with Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (98078-1-RR, Clone: 240793E8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98078-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98078-1-RR against Human CTLA4/CD152 were performed. The affinity constant is 60.2 pM.

CTLA-4/CD152 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98078-1-RR

Clone No.240793E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

CTLA4, 240793E8, CD152, CELIAC3, CTLA 4

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98078-1-RR targets CTLA-4/CD152 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0972

  Product name: Recombinant Human CTLA4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 36-161 aa of BC070162

  Sequence: KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD

• Full Name: cytotoxic T-lymphocyte-associated protein 4
• Calculated Molecular Weight: 223 aa, 25 kDa
• GenBank Accession Number: BC070162
• Gene Symbol: CTLA4
• Gene ID (NCBI): 1493
• RRID: AB_3672225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16410
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.