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CTNNA3 Recombinant monoclonal antibody, PBS Only (87022-1-PBS)

Filter: AllWBIHCIF-PAffinity

WB analysis using 87022-1-RR (same clone as 87022-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
IF Staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
IF Staining of rat heart using 87022-1-RR (same clone as 87022-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 87022-1-RR (same clone as 87022-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 87022-1-RR (same clone as 87022-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87022-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 87022-1-RR
Biolayer interferometry (BLl) kinetic assays of 87022-1-RR against Human CTNNA3 were performed. The affinity constant is 3.11 nM.

CTNNA3 Recombinant monoclonal antibody, PBS Only

Name: CTNNA3 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 87022-1-PBS

CTNNA3 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 87022-1-PBS

Clone No.252141F2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87022-1-PBS targets CTNNA3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag5008 Product name: Recombinant human CTNNA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC030646 Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL Predict reactive species
Full Name	catenin (cadherin-associated protein), alpha 3
Calculated Molecular Weight	100 kDa
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC030646
Gene Symbol	CTNNA3
Gene ID (NCBI)	29119
RRID	AB_3745280
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UI47
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).