CoraLite®594-conjugated CUL1 Monoclonal antibody

CUL1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6D10

Cat no : CL594-66978

Synonyms

CUL 1, CUL1, cullin 1

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-66978

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 CUL1 antibody (CL594-66978, Clone: 1B6D10 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL594-66978

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CUL1 (CL594-66978, Clone:1B6D10) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66978 targets CUL1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CUL1 fusion protein Ag28399
• Full Name: cullin 1
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC034318
• Gene Symbol: CUL1
• Gene ID (NCBI): 8454
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.