CUL1 Monoclonal antibody

CUL1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b


Human, Mouse, Rat







Cat no : 66978-1-Ig


CUL 1, CUL1, cullin 1

Tested Applications

Positive WB detected inHEK-293 cells, NCI-H1299 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66978-1-Ig targets CUL1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CUL1 fusion protein Ag28399
Full Name cullin 1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC034318
Gene Symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.


Product Specific Protocols
WB protocol for CUL1 antibody 66978-1-IgDownload protocol
IHC protocol for CUL1 antibody 66978-1-IgDownload protocol
IF protocol for CUL1 antibody 66978-1-IgDownload protocol
FC protocol for CUL1 antibody 66978-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols