CUL1 Monoclonal antibody

CUL1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1B6D10

Cat no : 66978-1-Ig

Synonyms

CUL 1, CUL1, cullin 1



Tested Applications

Positive WB detected inHEK-293 cells, NCI-H1299 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66978-1-Ig targets CUL1 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CUL1 fusion protein Ag28399
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1–3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 66978-1-IgDownload protocol
IHC protocol for CUL1 antibody 66978-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols