CXCR7-Specific Polyclonal antibody

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  WB analysis of K-562 using 14840-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 14840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14840-1-AP

  IP result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of mouse brain using 14840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCR7-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14840-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: K-562 cells, C6 cells, mouse brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14840-1-AP targets CXCR7-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_020311
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• RRID: AB_10646437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25106
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.