CXCR7-Specific Polyclonal antibody

CXCR7-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14840-1-AP

Synonyms

---

Validation Data Gallery

• 

  WB analysis of K-562 using 14840-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 14840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14840-1-AP

  IP result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

• 

  IHC staining of mouse brain using 14840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: K-562 cells, C6 cells, mouse brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14840-1-AP targets CXCR7-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_020311
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• RRID: AB_10646437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.

Related Products

CXCR7 Polyclonal antibody

20423-1-AP

CXCR7 Monoclonal antibody

60216-1-Ig

CXCL11 Polyclonal antibody

10707-1-AP

CXCR4 Polyclonal antibody

11073-2-AP

CXCR4 Monoclonal antibody

60042-1-Ig

HIC1 Polyclonal antibody

24949-1-AP

CXCR3B-specific Monoclonal antibody

60065-1-Ig

Beta Arrestin 1 Polyclonal antibody

15361-1-AP

Beta Arrestin 2 Polyclonal antibody

10171-1-AP