Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 29 publications below |
| IHC | See 2 publications below |
Product Information
24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19374 Product name: Recombinant human CYP7B1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 96-403 aa of BC136574 Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD Predict reactive species |
| Full Name | cytochrome P450, family 7, subfamily B, polypeptide 1 |
| Calculated Molecular Weight | 506 aa, 58 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC136574 |
| Gene Symbol | CYP7B1 |
| Gene ID (NCBI) | 9420 |
| RRID | AB_2879780 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75881 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| IHC protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| WB protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism | ||
Cell Physiol Biochem Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. | ||
Mol Nutr Food Res The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation | ||
J Nutr Biochem Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice. | ||
Biochem Pharmacol Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity. | ||
J Lipid Res Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition. |
