Calbindin-D28k Monoclonal antibody

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  WB analysis using 66394-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66394-1-Ig

  Human brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66394-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 66394-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66394-1-Ig (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human kidney using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green).

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  IF Staining of rat cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 66394-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 66394-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Calbindin-D28k Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 66394-1-Ig

Clone No.1F8B9

Publications(12)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

CALB1, Calbindin, 1F8B9, CALB, calbindin 1, 28kDa

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Tested Applications

• Positive WB detected in: rabbit brain tissue, PC-12 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue
• Positive IHC detected in: rat cerebellum tissue, human kidney tissue, human small intestine tissue, human brain tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue, human kidney tissue, rat cerebellum tissue
• Positive IF-Fro detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:8000-1:32000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Product Information

66394-1-Ig targets Calbindin-D28k in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calbindin-D28k fusion protein Ag5861
• Full Name: calbindin 1, 28kDa
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC006478
• Gene Symbol: Calbindin
• Gene ID (NCBI): 793
• RRID: AB_2881769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05937
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); Integrative Single-Cell Transcriptomics and Epigenomics Mapping of the Fetal Retina Developmental Dynamics; Authors - Ruonan Li
mouse	IF	Cell Death Differ; The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.; Authors - Fei Wang
mouse	IF	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	IF	Int Immunopharmacol; Clinical significance of Interleukin 17 receptor E in diabetic nephropathy; Authors - Jingjing Wang
rat	IF	Front Neurosci; Rodent Area Prostriata Converges Multimodal Hierarchical Inputs and Projects to the Structures Important for Visuomotor Behaviors.; Authors - Chang-Hui Chen
rat	IF	Neurosci Lett; Retinoic acid supplementation ameliorates motor incoordination via RARα-CBLN2 in the cerebellum of a prenatal valproic acid-exposed rat autism model; Authors - Binlin Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (08-08-2024)

Calbindin (green) worked perfectly marking some of my neurons (red) in human brain ffpe tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Calbindin-D28k Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66394-1-Ig)