Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig







Cat no : 66394-1-Ig


CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, Calbindin-D28k, D 28K

Tested Applications

Positive WB detected inpig cerebellum tissue, PC-12 cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inrat cerebellum tissue, human kidney tissue, human small intestine tissue, human brain tissue, mouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue, human kidney tissue, PC-12 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

66394-1-Ig targets Calbindin-D28k in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.


Product Specific Protocols
WB protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
IHC protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
IF protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Differ

The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.

Authors - Fei Wang

Front Neurosci

Rodent Area Prostriata Converges Multimodal Hierarchical Inputs and Projects to the Structures Important for Visuomotor Behaviors.

Authors - Chang-Hui Chen