Calbindin-D28k Monoclonal antibody

Calbindin-D28k Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F8B9

Cat no : 66394-1-Ig

Synonyms

CAB27, CALB, CALB1, Calbindin, calbindin 1, 28kDa, Calbindin D28, D 28K



Tested Applications

Positive WB detected inpig cerebellum tissue, PC-12 cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman brain tissue, human kidney tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue, PC-12 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66394-1-Ig targets Calbindin-D28k in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Calbindin-D28k fusion protein Ag5861
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC006478
Gene symbol CALB1
Gene ID (NCBI) 793
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
WB protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
IHC protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
IF protocol for Calbindin-D28k antibody 66394-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols