Calpain 6 Polyclonal antibody, PBS Only

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• Calpain 6 Polyclonal antibody, PBS Only (10120-1-PBS)

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  WB analysis using 10120-1-AP (same clone as 10120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10120-1-PBS in a different storage buffer formulation.

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  WB analysis using 10120-1-AP (same clone as 10120-1-PBS)

  Mouse placenta tissue was subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10120-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 10120-1-AP (same clone as 10120-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10120-1-AP (Calpain 6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10120-1-PBS in a different storage buffer formulation.

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  IP experiment of human placenta using 10120-1-AP (same clone as 10120-1-PBS)

  IP result of anti-Calpain 6 (IP:10120-1-AP, 4ug; Detection:10120-1-AP 1:300) with human placenta tissue lysate 1520ug. This data was developed using the same antibody clone with 10120-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10120-1-AP (same clone as 10120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10120-1-AP (Calpain 6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10120-1-PBS in a different storage buffer formulation.

Calpain 6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10120-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

10120-1-PBS targets Calpain 6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0160

  Product name: Recombinant human CAPN6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 67-360 aa of BC000730

  Sequence: NISNHQLTQGRLGHKPMVSAFSCLAVQESHWTKTIPNHKEQEWDPQKTEKYAGIFHFRFWHFGEWTEVVIDDLLPTINGDLVFSFSTSMNEFWNALLEKAYAKLLGCYEALDGLTITDIIVDFTGTLAETVDMQKGRYTELVEEKYKLFGELYKTFTKGGLICCSIESPNQEEQEVETDWGLLKGHTYTMTDIRKIRLGERLVEVFSAEKVYMVRLRNPLGRQEWSGPWSEISEEWQQLTASDRKNLGLVMSDDGEFWMSLEDFCRNFHKLNVCRNVNNPIFGRKELESVLGCW

• Full Name: calpain 6
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 66-75 kDa
• GenBank Accession Number: BC000730
• Gene Symbol: Calpain 6
• Gene ID (NCBI): 827
• RRID: AB_2290829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6Q1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.