Calumenin Polyclonal antibody, PBS Only

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• Calumenin Polyclonal antibody, PBS Only (17804-1-PBS)

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• KD/KO Validated

Calumenin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17804-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CALU, Crocalbin, IEF SSP 9302

• 

  WB analysis of HEK-293 using 17804-1-AP (same clone as 17804-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 17804-1-AP (same clone as 17804-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 17804-1-AP (same clone as 17804-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 17804-1-AP (same clone as 17804-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 17804-1-AP (same clone as 17804-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17804-1-AP (same clone as 17804-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 17804-1-AP (same clone as 17804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 17804-1-AP (same clone as 17804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17804-1-AP (same clone as 17804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17804-1-AP (same clone as 17804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 17804-1-AP (same clone as 17804-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (17804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17804-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

17804-1-PBS targets Calumenin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12073

  Product name: Recombinant human CALU protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC013383

  Sequence: MDLRQFLMCLSLCTAFALSKPTEKKDRVHHEPQLSDKVHNDAQSFDYDHDAFLGAEEAKTFDQLTPEESKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFGEALVRHDEF

• Full Name: calumenin
• Calculated Molecular Weight: 315 aa, 37 kDa
• Observed Molecular Weight: 47 kDa, 57 kDa
• GenBank Accession Number: BC013383
• Gene Symbol: Calumenin
• Gene ID (NCBI): 813
• RRID: AB_2069964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43852
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.