Tested Applications
| Positive WB detected in | rat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue |
| Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK Predict reactive species |
| Full Name | troponin T type 2 (cardiac) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC002653 |
| Gene Symbol | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3085051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P45379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| IHC protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| WB protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cardiac Troponin T Monoclonal antibody (4E2D8), catalog number 68300-1-Ig, has 30 citations. These appear in journals including Nature Communications, Circulation, Nature Genetics, Advanced Materials, ACS Nano, Cardiovascular Diabetology, Advanced Science, Journal of Molecular and Cellular Cardiology, iScience, International Journal of Cardiology, European Journal of Pharmacology, and others. Research fields span cardiac disease, myocardial infarction, diabetic cardiomyopathy, sepsis-induced cardiac injury, ferroptosis, ischemia/reperfusion injury, heart failure, angiogenesis, and cardiac inflammation.
| Species | Application | Title |
|---|---|---|
Circulation Aberrant Phase Separation of Endothelial MAML1 Causes Congenital Heart Disease by Suppressing Notch Activity | ||
Adv Mater DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation | ||
Nat Genet Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activation. | ||
Nat Commun Exercise-induced histone lactylation in monocyte-derived macrophages restores cardiac immune homeostasis and function in sepsis-induced cardiomyopathy. | ||
Nat Commun LTBP4 deficiency inhibits NLRP3 inflammasome activation in cardiomyocytes and attenuates heart failure in male mice |
Reviews
What customers say
Both reviewers rated the antibody 5 stars. One used it for immunohistochemistry on human iPSC-derived organoids at a 1:500 dilution with overnight incubation in PBST with 5% donkey serum, reporting it worked well. The other tested it on chicken heart paraffin sections at 1:200–1:400 for both Western blot and immunofluorescence, noting specific positive IF staining and clear bands at the correct molecular weight. Overall, users found the antibody specific and reliable across multiple applications and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
REFIK (Verified Customer) (03-05-2026) | Used for human derived iPSCs derived organoids, works well with 1:500 dilution for overnight incubation in 1x PBST in 5% Donkey Serum
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Josh (Verified Customer) (04-24-2025) | Specific antibody, positive IF-P staining in paraffin embedded chick heart tissue and clear bands at the correct molecular weight when running a Western blot.
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