CoraLite®650-conjugated Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®650 Fluorescent Dye

Cat no : CL650-10380

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6



Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10380-1-AP

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL650-10380 targets Caspase 9/p35/p10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 9/p35/p10 fusion protein Ag0404
Full Name caspase 9, apoptosis-related cysteine peptidase
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 35 kDa
GenBank accession numberBC002452
Gene symbol CASP9
Gene ID (NCBI) 842
Conjugate CoraLite®650 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9.

Protocols

Product Specific Protocols
FC protocol for CL650 Caspase 9/p35/p10 antibody CL650-10380Download protocol
Standard Protocols
Click here to view our Standard Protocols