Mouse Cd74 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP02137-1

  Sandwich ELISA standard curve of MP02137-1, Mouse CD74 Recombinant Matched Antibody Pair - PBS only. 85802-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2716. 85802-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP02137-1

  RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD74, and measured 14.17 ng/mL.

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  Sample test of MP02137-1

  The mean CD74 concentration was determined to be 856.01 ng/mL in mouse thymus tissue extract based on a 5.80 mg/mL extract load and 789.39 ng/mL in mouse spleen tissue extract based on a 8.50 mg/mL extract load.

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  Sample test of MP02137-1

  Serum of sixteen mice was measured. The CD74 concentration of detected samples was determined to be 21.51 pg/mL with a range of 12.61-42.94 pg/mL.

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  Cytometric bead array standard curve of MP02137-2

  Cytometric bead array standard curve of MP02137-2, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-3-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.

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  Cytometric bead array standard curve of MP02137-1

  Cytometric bead array standard curve of MP02137-1, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-1-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.

Cd74 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85802-2-PBS

Clone No.250109B11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

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Product Information

85802-2-PBS targets Cd74 as part of a matched antibody pair:

MP02137-1: 85802-1-PBS capture and 85802-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP02137-2: 85802-3-PBS capture and 85802-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cd74 fusion protein Eg2716
• Full Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001042605.1
• Gene Symbol: Cd74
• Gene ID (NCBI): 16149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04441-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.