PE-conjugated Cleaved Caspase 3/P17/P19 Monoclonal antibody

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• PE-conjugated Cleaved Caspase 3/P17/P19 Monoclonal antibody (PE-68773)

Cleaved Caspase 3/P17/P19 Monoclonal Antibody for IF/ICC

Cat No. PE-68773

Clone No.2F7B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

CASP3, Caspase3, CASP 3, CASP-3, Caspase 3

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  IF Staining of Jurkat using PE-68773

  Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using PE Cleaved Caspase 3/P17/P19 antibody (PE-68773, Clone: 2F7B8 ) at dilution of 1:100.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF/ICC detected in: Staurosporine treated Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-68773 targets Cleaved Caspase 3/P17/P19 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 17 kDa, 19 kDa
• GenBank Accession Number: NM_032991
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P42574
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).